Journal
BIOTECHNIQUES
Volume 50, Issue 3, Pages 187-189Publisher
FUTURE SCI LTD
DOI: 10.2144/000113628
Keywords
Magnetofection; transfection; neural stem cells; NSC; NeuroMag; neuronal differentiation
Funding
- European Union [LSHM-CT-2006-037315]
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Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection efficiency and can be used for deciphering the genetic control of neural cell differentiation.
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