Journal
BIOTECHNIQUES
Volume 51, Issue 2, Pages 111-+Publisher
FUTURE SCI LTD
DOI: 10.2144/000113719
Keywords
PLA; GPCR; oligomers
Funding
- National Institutes of Health [NIH] [DA022413, MH054137, F32NS064732]
- Research Foundation for Mental Hygiene
- Lieber Center for Schizophrenia Research and Treatment
- European Molecular Biology Organization (EMBO)
- Basque Country Government
- Fondation pour la Recherche Medicale
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The existence of G protein-coupled receptor (GPCR) dimers and/or oligomers has been demonstrated in heterologous systems using a variety of biochemical and biophysical assays. While these interactions are the subject of intense research because of their potential role in modulating signaling and altering pharmacology, evidence for the existence of receptor interactions in vivo is still elusive because of a lack of appropriate methods to detect them. Here, we adapted and optimized a proximity ligation assay (PLA) for the detection in brain slices of molecular proximity of two antigens located on either the same or two different GPCRs. Using this approach, we were able to confirm the existence of dopamine D2 and adenosine A2A receptor complexes in the striatum of mice ex vivo.
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