Journal
BIOTECHNIQUES
Volume 51, Issue 3, Pages 185-189Publisher
BIOTECHNIQUES OFFICE
DOI: 10.2144/000113738
Keywords
H2AX; gamma H2AX; H2AX-foci; cancer; irradiation; immunofluorescence staining; spotting/arraying
Funding
- Swedish Cancer Society
- Swedish Research Council
- Sahlgrenska University Hospital
- Swedish Pain Foundation (SSF)
- King Gustav V Jubilee Clinic Cancer Research Foundation
- Assar Gabrielsson Cancer Research Foundation
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Immunofluorescence quantification of gamma H2AX foci is a powerful approach to quantify DNA double-strand breaks induced by cancer therapy or accidental exposure to ionizing radiation. Here we report a modification to the gamma H2AX immunofluorescence labeling method, whereby cells are stained in-solution before being spotted and fixed onto microscope slides. Our modified method allows arraying of 16 patient samples/slide ready for foci counting in 2 h and demonstrated reliably detection of gamma H2AX foci in mononuclear cells prepared from patients who had undergone radiation therapy.
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