Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1424, Issue -, Pages 18-26Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2015.11.004
Keywords
Cu2+-EDTA-Fe3O4 particles; Adsorption; Hemoglobin; Removal; Blood
Funding
- National Natural Science Foundation of China [21175048]
- Natural Science Foundation of Guangdong Province, China [2015A030311013]
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In this report, the Cu2+-immobilized magnetic particles were prepared by a facile route and they were used as adsorbents for removal of high abundance of hemoglobin in blood based on immobilized metal affinity chromatography. Ethylenediaminetetraacetic acid modified magnetic particles (EDTA-Fe3O4) were first synthesized through a one-pot solvothermal method and then charged with copper ions. The as-prepared Cu2+-EDTA-Fe3O4 particles were characterized by Fourier transform infrared spectrometry, scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray spectroscopy, vibrating sample magnetometry and zeta potential. Factors affecting the adsorption of bovine hemoglobin on Cu2+-EDTA-Fe3O4 particles (including contact time, solution pH, ionic strength and initial concentration of protein) were investigated. The adsorption process followed a pseudo-second-order kinetic model and the adsorption equilibrium could be achieved in 60 min. The adsorption isotherm data could be well described by a Langmuir model and the maximum adsorption capacity was 1250 mg g(-1). The as-prepared particles showed high efficiency and excellent selectivity for removal of hemoglobin from bovine and human blood. The removal process integrated the selectivity of immobilized metal affinity chromatography and the convenience of magnetic separation. The results demonstrated that Cu2+-EDTA-Fe3O4 particles had potential application in removal of abundant histidine-rich proteins in biomedical diagnosis analysis. (C) 2015 Elsevier B.V. All rights reserved.
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