Journal
BIOTECHNIQUES
Volume 48, Issue 4, Pages 317-319Publisher
FUTURE SCI LTD
DOI: 10.2144/000113403
Keywords
cytosine hydroxymethylation; cytosine methylation; hmC; epigenetics; transcriptional repression
Funding
- Medical Research Council
- Breakthrough Breast Cancer
- Medical Research Council [MC_U127574433] Funding Source: researchfish
- MRC [MC_U127574433] Funding Source: UKRI
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DNA cytosine methylation (5mC) is highly abundant in mammalian cells and is associated with transcriptional repression. Recently, hydroxymethylcytosine (hmC) has been detected at high levels in certain human cell types; however, its roles are unknown. Due to the structural similarity between 5mC and hmC, it is unclear whether 5mC analyses can discriminate between these nucleotides. Here we show that 5mC and hmC are experimentally indistinguishable using established 5mC mapping methods, thereby implying that existing 5mC data sets will require careful re-evaluation in the context of the possible presence of hmC. Potential differential enrichment of 5mC and hmC DNA sequences may be facilitated using a 5mC monoclonal antibody.
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