Journal
BIOTECHNIQUES
Volume 49, Issue 6, Pages 898-+Publisher
FUTURE SCI LTD
DOI: 10.2144/000113556
Keywords
mRNA-Seq; transcriptome; gene expression; high-throughput sequencing
Funding
- Morgridge Institute for Research
- University of Wisconsin Foundation
- Tactics II Stem Cell Ventures
Ask authors/readers for more resources
Preparation of an Illumina sequencing library for gene expression analysis (mRNA-Seq) requires microgram amounts of starting total RNA or PCR-based amplification. Here we describe a protocol based on T7 linear RNA amplification that does not introduce significant bias, requires only 10 ng total RNA, and generates a directional, fully representative, whole-transcript mRNA-Seq Illumina library that is highly consistent across over three orders of magnitude of input RNA.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available