Journal
BIOTECHNIQUES
Volume 45, Issue 2, Pages 179-+Publisher
INFORMA HEALTHCARE
DOI: 10.2144/000112889
Keywords
-
Funding
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (NIH/NIAID) [U01A1070276]
Ask authors/readers for more resources
One limitation that accounts in part for the scarcity of commercially available diagnostic microarrays is the expense associated with fluorescence detection, Here we present a colorimetric method based on photopolymerization as an on-chip signal amplification technique. Proof of principle experiments are detailed and followed by the use of a simple influenza microarray to demonstrate the technique for the first time with clinical samples. The advantages of this new technique include rapid (<5 min) signal amplification similar to 10(5)) in ambient conditions for both DNA and protein microarrays, low reagent cost (<$1 per assay), visual or inexpensive detection, and signal preservation for at least two years under ambient conditions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available