Journal
BIOTECHNIC & HISTOCHEMISTRY
Volume 85, Issue 2, Pages 127-131Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/10520290903149604
Keywords
apoptosis; flow cytometry; green fluorescent protein; histone; mitosis
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Funding
- NIH [CA92309, CA9230904S1]
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Green fluorescent protein (GFP) has become a powerful tool for monitoring the expression of transfected genes by flow cytometry including GFP-tagged histones for tracking chromatin and elucidating histone function. We describe here a method for simultaneous detection of three nucleus-localized signals: a GFP-tagged histone, DNA content and detection of phosphorylated histone H3, which labels mitotic cells. We also demonstrate another application of this method for simultaneous detection of a GFP-tagged histone, DNA content, and cleaved caspase-3.
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