Journal
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
Volume 16, Issue -, Pages 669-676Publisher
ELSEVIER
DOI: 10.1016/j.bcab.2018.09.024
Keywords
Xylanase; Solid state fermentation; Sugarcane bagasse; Palm kernel cake; Environmental sources
Categories
Funding
- Universiti Sains Malaysia [304/PTEKIND/6312113]
- School of Industrial Technology
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Xylanase is an enzyme that degrades hemicelluloses. The goal of the present study was to investigate the production of xylanase by fungi via the solid substrate fermentation (SSF) process using sugarcane bagasse and palm kernel cake as substrate. During preliminary screening, 15 potential xylanase producer strains were isolated from 9 different environmental sources. Eight out of the 15 isolates produced positive results in the submerged fermentation process and clear-zone formation analysis. The decayed wood isolate had the highest xylanase activity, at 154.2 U/g in the secondary screening process. This isolate, identified as Aspergillus niger, was selected for further study. The maximum xylanase activity of 456.4 U/g was obtained under optimized conditions of 6 g of substrate, 75% (w/w) moisture content, temperature of 34 degrees C, and initial medium of pH 6 supplemented with 2% (w/v) glucose and 0.25 mM manganese ions. This activity was 1.5-fold higher than that reported in previous studies. These results illustrate that an increased xylanase yield can be obtained via SSF using agro-industry residual products such as palm kernel cake and sugarcane bagasse as substrates, which would reduce the overall cost of producing xylanase.
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