Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 407, Issue 16, Pages 4697-4708Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-015-8673-7
Keywords
Imaging mass spectrometry; Lipidomics; Colon; MALDI; LC/MS; Analytical methods
Funding
- Institute of Health Carlos III (Ministerio de Economia y Competitividad)
- EC (European Regional Development Fund, ERDF) [CP12/03338]
- UPV/EHU [UFI 11/23]
- Basque Government (SAIOTEK)
- Miguel Servet program of the Institute of Health Carlos III
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Imaging mass spectrometry is becoming a reference technique in the field of lipidomics, due to its ability to map the distribution of hundreds of species in a single run, along a tissue section. The next frontier is now achieving increasing resolution powers to offer cellular (or even sub-cellular) resolution. Thus, the new spectrometers are equipped with sophisticated optical systems to decrease the laser spot to < 30 mu m. Here, we demonstrate that by using the correct matrix (i.e., a matrix that maximizes ion detection and forms small crystals) and a careful preparation, it is possible to achieve resolutions of similar to 5-10 mu m, even with spectrometers equipped with non-optimal optics, which produces laser spots of 50 mu m or even larger. As a proof of concept, we present images of distributions of lipids, both in positive and negative ion mode, over human colon endoscopic sections, recorded using 2-mercaptobenzothiazole for positive ion mode and 2,5-diaminonaphtalene for negative ion mode and an LTQ-Orbitrap XL, equipped with a matrix-assisted laser desorption ionization (MALDI) source that produces astigmatic laser spots.
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