Journal
BIOSENSORS & BIOELECTRONICS
Volume 123, Issue -, Pages 51-58Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.09.079
Keywords
Bimetallic ZnZr-metal organic frameworks; Electrochemical aptasensor; Detection of protein tyrosine kinase-7; MOF-on-MOF hybrids
Categories
Funding
- Programs for the National Natural Science Foundation of China (NSFC) [81601082, 81371363, U1604127, U1704256]
- Innovative Technology Team of Henan Province Technology of China [CXTD2014042]
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Fabricating novel bimetallic metal organic framework (MOF) architectures and exploiting them as aptasensor scaffolds for detecting diverse analytes, especially cancer markers, have aroused widespread research attention. Herein, we report a novel strategy for obtaining ZnZr bimetallic MOFs via the MOF-on-MOF method and exploit them as an aptasensor platform for detecting the cancer marker protein tyrosine kinase-7 (PTK7). Basic characterizations reveal that the chemical structure, crystalline properties, and surface functionality of bimetallic Zn-Zr-MOFs can be modulated by changing the order of addition of metal precursors and organic ligands. The Zn-MOF-on-Zr-MOF hybrid exhibits a hierarchically decussated foliace, whereas Zr-MOF-on-Zn-MOF demonstrates a multilayered nanosheet structure. The electrochemical results reveal that Zr-MOF facilitates aptamer strand immobilization, whereas the Zn-MOF stabilizes the G-quadruplex formed by aptamer strands and PTK7. The Zn-MOF-on-Zr-MOF-based aptasensor outperforms the Zr-MOF-on-Zn-MOF-based one, providing ultralow detection limits of 0.84 and 0.66 pg mL(-1), as obtained by electrochemical impedance spectroscopy and differential pulse voltammetry, respectively, within the PTK7 concentration range of 1.0 pg mL(-1) to 1.0 ng mL(-1). The proposed Zn-MOF-on-Zr-MOF-based aptasensor exhibits high selectivity in the presence of various interferences, good stability, reproducibility, and acceptability in human serum. The proposed strategy provides a new approach for fabricating ultrasensitive and selective bimetallic MOFs-based aptasensors and contributes to efforts to broaden their applications in early cancer diagnosis.
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