4.8 Article

A dual-channel homogeneous aptasensor combining colorimetric with electrochemical strategy for thrombin

Journal

BIOSENSORS & BIOELECTRONICS
Volume 120, Issue -, Pages 15-21

Publisher

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.08.031

Keywords

Dual-channel; Homogeneous aptasensor; Electrochemical; Colorimetric; Hydroxyapatite nanoparticles

Funding

  1. National Natural Science Foundation of China [21645007, 21475071]
  2. Taishan Scholar Program of Shandong Province [ts201511027]
  3. Natural Science Foundation of Shandong [ZR2016BM21]

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In this protocol, a dual-channel homogeneous aptasenor was proposed for protein molecule determination, employing thrombin as target analyte. The colorimetric and electrochemical transducers were combined in a single analytical system for signal readout. In this dual-channel sensing strategy, the G-quadruplex sequence was released and incorporated with hemin to form DNAzyme for naked-eye colorimetric detection. Meanwhile, the hydroxyapatite nanoparticle as signal probe was combined with magnetic nanoparticles to construct sandwich type structure for generating the electrochemical current when thrombin was present in solution. By introducing two kinds of reporter probes and transducers, this dual-channel sensor produced two different kinds of signal to improve the analytical accuracy and diversity. The results revealed that the dual-channel sensor achieved the quantatitive determination of thrombin with low limit of detection (0.40 fM) and wide range (0.1 fM to 1 nM), which offer a promise for rapid and accurate detection of biomolecule.

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