Journal
BIOSENSORS & BIOELECTRONICS
Volume 59, Issue -, Pages 140-144Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.03.021
Keywords
Planar Hall magnetoresistive sensor; Aptasensor; Thrombin; Sandwich
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Funding
- SMBA 2012 program [C0021020]
- BioNano Health-Guard Research Center - Ministry of Science, ICT & Future Planning (MSIP) of Korea as Global Frontier Projec [H-GUARD_2013M3A6B2078]
- Korea Evaluation Institute of Industrial Technology (KEIT) [C0021020] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The use of aptamer-based assays is an emerging and attractive approach in disease research and clinical diagnostics. A sensitive aptamer-based sandwich-type sensor is presented to detect human thrombin using a planar Hall magnetoresistive (PHR) sensor in cooperation with superparamagnetic labels. A PHR sensor has the great advantages of a high signal-to-noise ratio, a small offset voltage and linear response in the low-field region, allowing it to act as a high-resolution biosensor. In the system presented here, the sensor has an active area of 50 mu m x 50 mu m with a 10-nm gold layer deposited onto the sensor surface prior to the binding of thiolated DNA primary aptamer. A polydimethylsiloxane well of 600-mu m radius and 1-mm height was prepared around the sensor surface to maintain the same specific area and volume for each sensor. The sensor response was traced in real time upon the addition of streptavidin-functionalized magnetic labels on the sensor. A linear response to the thrombin concentration in the range of 86 pM-8.6 mu M and a lower detection limit down to 86 pM was achieved by the proposed present method with a sample volume consumption of 2 mu l. The proposed aptasensor has a strong potential for application in clinical diagnosis. (C) 2014 Elsevier B.V. All rights reserved.
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