Journal
BIOSENSORS & BIOELECTRONICS
Volume 60, Issue -, Pages 118-123Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.03.075
Keywords
Pebrine disease; Electrochemical immunosensor; Electrocatalytic; Hemin/G-quadruplex; Pt@Pb nanowires; Nosema bombycis
Categories
Funding
- NNSF of China [21075100, 21275119]
- Ministry of Education of China [708073]
- Natural Science Foundation of Chongqing City [CSTC-2011BA7003, CSTC-2010BB4121]
- State Key Laboratory of Silkworm Genome Biology [sklsgb2013012]
- Fundamental Research Funds for the Central Universities [XDJK2013A008, XDJK2014C138C]
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In this work, an ultrasensitive electrochemical immunosensor for detecting the Pebrine disease related spore wall protein of Nosema bombycis (sWP N.b) was fabricated based on the amplification of hemin/G-quadruplex functionalized Pt@Pd nanowires (Pt@PdNVVs). The synthesized Pt@PdNWs possessed large surface area, which could effectively improve the immobilization amount of hemin/G-quadruplex DNAzyme concatamers produced via hybridization chain reaction (HCR). In the presence of SWP N.b, the hemin/G-quadruplex labeled Pt@PdNWs bioconjugations was captured on electrode surface and thus obtained electrochemical signal. After the addition of NADH into the electrolytic cell, hemin/G-quadruplex firstly acted as an NADH oxidase to locally produce H2O2 in the presence of dissolved O-2. Then, the generated H2O2 would be quickly reduced via hemin/G-quadruplex as a horseradish peroxidase mimicking (HRP-mimicking) DNAzyme, which finally promoted the self-redox reaction of hemin/G-quadruplex and a greatly enhanced electrochemical signal was obtained. Furthermore, Pt@PdNINS with excellent electrocatalytic performance could also amplify electrochemical signal. With these amplification factors, the electrochemical immunosensor exhibited a wide linear range from 0.001 ng mL(-1) to 100 ng mL(-1) with a detection limit (LOD) of 0.24 pg mL(-1), providing a new promise for the diagnosis of Pebrine disease. (C) 2014 Elsevier B.V. All rights reserved.
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