Journal
BIOSENSORS & BIOELECTRONICS
Volume 61, Issue -, Pages 51-56Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.04.030
Keywords
Fluorescence; ATP; Aptamer; DNA; Circular amplification
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Funding
- National Natural Science Foundation of China [21275086, 21227008]
- National Basic Research Program of China [2010CB732404]
- Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT)
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In this work, isothermal circular strand-displacement polymerization amplification assay is developed for highly specific and sensitive detection of adenosine triphosphate (ATP). The amplification process consists of circular common target molecule-displacement polymerization (CCDP) and circular nucleic acid strand-displacement polymerization (CNDP). In the presence of ATP, the complementary strand was released from the aptamer by the target recognition of ATP, and catalyzed the subsequent cycle reaction. With the polymerase and primer, the displaced target triggers the process of CCDP. With the involvement of nicking endonuclease, the released complementary strand triggers the CNDP. Combined CCDP with CNDP, the exponentially produced fluorescence probes are obtained, achieving a detection limit of ATP as low as 2.6 x 10(-10) M. Moreover, the proposed strategy exhibits an excellent specificity and is successfully applied in real sample assay which demonstrates potential application in practical samples. (C) 2014 Elsevier B.V. All rights reserved.
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