A resorufin-based colorimetric and fluorescent probe for live-cell monitoring of hydrazine

We report a novel colorimetric and red-emitting fluorescent probe for hydrazine detection based on resorufin platform. This OFF-ON fluorescent probe shows a large (117 nm) red-shifted absorption spectrum and the color changes from colorless to red upon addition of hydrazine in the aqueous solution, which can serve as a naked-eye probe for hydrazine. Moreover, this probe also shows a significant fluorescence increase (similar to 16 folds) and excellent linear relationship at physiological pH. Utilizing this sensitive and selective probe, we have successfully detected hydrazine in living cells. Crown Copyright (C) 2014 Published by Elsevier B.V. All rights reserved.