Journal
BIOSENSORS & BIOELECTRONICS
Volume 61, Issue -, Pages 344-350Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.05.049
Keywords
DNA sensor; Metal-enhanced fluorescence; Silver nanoparticle; Zinc oxide quantum dots
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Funding
- National Natural Science Foundation of China [51273084, 21277058]
- Natural Science Foundation of Shandong Province, China [ZR2012BZ002, ZR201113Q019]
- Technology Development Plan of Shandong Province, China [2012GGB011813]
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We reported here the preparation of dioscorea batatas bean-like silver nanoparticles (AgNPs) and the unique structure provided the AgNPs good localized surface plasmon resonance (LSPR) property. In addition, zinc oxide quantum dots (ZnO QDs) were also synthesized and found with good fluorescent property. Furthermore, the ZnO QDs decorated exfoliated graphene oxide (EGO-ZnO) was prepared via electrostatic interaction. The named nanomaterials were applied in a LSPR-induced fluorescent DNA sensor. To fabricate the DNA sensor, the EGO-ZnO was modified on the silica glass as the supporter for the capture probe ssDNA, and the complementary ssDNA was labeled on the surface of the AgNPs. After the hybridization step by step, the AgNPs was fastened on the surface of the EGO-ZnO, and the fluorescent intensity of the EGO-ZnO increased as a result. The prepared DNA sensor enabled the target ssDNA to be detected in the concentration range of 10(-19)-10(-14) M, and the limit of detection was 4.3 x 10(-20) M. (C) 2014 Elsevier B.V. All rights reserved.
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