Journal
BIOSENSORS & BIOELECTRONICS
Volume 61, Issue -, Pages 410-416Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.05.052
Keywords
Hemin/G-quadruplexes; Horseradish peroxidase-mimicking enzyme; Potassium ions; Au nanoparticles; Bioelectrocatalysis
Categories
Funding
- National Natural Science Foundation of China [21073001, 21005001, 21371007]
- Anhui Provincial Natural Science Foundation [1208085QB28, 1408085J03]
- Natural Science Foundation of Anhui [KJ2012A139]
- Program for Innovative Research Team at Anhui Normal University
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Sensitive and selective sensors need to be explored to detect the physiological potassium level due to its important role in the living organisms. In the present system, a novel electron transfer mediator actuated electrocatalytical biosensor was demonstrated to assay K+ based on the conformational change of DNA. With the hybridization between the complementary bases and the self-folding of guanine-rich nucleic acid sequence, the horseradish peroxidase-mimicking enzyme (HRP-DNAzyme) was formed and brought to approach the ferrocene (Fc) unit on Au nanoparticles (AuNPs). Thus, in the system, Fc unit acted as the relay, stimulating the electrical contact of HRP-DNAzyme with the electrode to obtain the bioelectrocatalyze reduction signal. Under the Fc actuated catalysis of HRP-DNAzyme and amplification of Au nanoparticles, the obtained biosensor exhibited a sensitive detection for K+. A satisfying result of a wide linear range and low detection limit were obtained with the novel electrocatalytical biosensor which was then applied in real samples. (C) 2014 Elsevier B.V. All rights reserved.
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