Journal
BIOSENSORS & BIOELECTRONICS
Volume 62, Issue -, Pages 214-220Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.06.045
Keywords
Heme proteins; Honeybee silk; Optical biosensors; Coiled-coil proteins
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In this work we investigate the use of coiled-coil silk proteins, produced in recombinant Escherichia coli, as a new material for immobilizing biosensors. Myoglobin was embedded in transparent honeybee silk protein films. Immobilized myoglobin maintained a high affinity for nitric oxide (K-D NO = 52 mu M) and good sensitivity with a limit of detection of 5 mu M. The immobilized myoglobin-silk protein film was stable and could be stored as a dry film at room temperature for at least 60 days. The effect of immobilization on the structure of myoglobin was fully investigated using UV/visible, Fourier Transform Infrared and Raman spectroscopy, which indicated a weakening in the strength of the iron-histidine bond. This study demonstrates that recombinant coiled-coil silk proteins provide a safe and environmentally friendly alternative to sol-gels for stabilizing heme proteins for use as optical biosensors. (C) 2014 The Authors. Published by Elsevier B.V.
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