Journal
BIOSENSORS & BIOELECTRONICS
Volume 51, Issue -, Pages 413-420Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.07.066
Keywords
Photoelectrochemical biosensor; ZnO/graphene; S6 aptamers; SK-BR-3 cancer cells; Indium tin oxide device
Categories
Funding
- Natural Science Research Foundation of China [21277058, 21175058]
- Natural Science Foundation of Shandong Province, China [ZR2012BZ002, ZR2011BQ019, ZR2011EL029]
Ask authors/readers for more resources
ZnO/graphene (ZnO/G) composite and S6 aptamer were employed to sensitive photoelectrochemical (PEC) strategy for the specific detection of SK-BR-3 cancer cells based on a portable indium tin oxide microdevice. ZnO/G composite was synthesized using a facile ultrasonic method, and then applied to improve the PEC performance due to the unique hollow structure of ZnO naospheres and the superior properties of graphene. Subsequently, S6 aptamer was applied to this specific detection of SK-BR-3 cancer cells. And the concentration of SK-BR-3 cells was measured with a low detection limit of 58 cells mL(-1) and a wide linear range of 1 x 10(2) - 1 x 10(6) cells mL(-1), through the decrease in photocurrent intensity resulting from the increase in steric hindrances when specifically recognized with S6 aptamers. Excellent discrimination against target and analogous cells was demonstrated, indicating the high selectivity of the proposed cell sensor. Our work also demonstrated a sensitive, stable and low cytotoxicity approach for early and accurate detection of cancer cells. (C) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available