Journal
BIOSENSORS & BIOELECTRONICS
Volume 53, Issue -, Pages 245-249Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.09.055
Keywords
DNAzyme; Lead; Strand displacement amplification; Fluorescence; Exonuclease III
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Funding
- 100 Talents program of the Chinese Academy of Sciences
- National Basic Research Program of China (973 Program) [2011CB911002]
- National Natural Science Foundation of China [21075119, 91027036, 21275139]
- Pillar Program of Changchun Municipal Bureau of Science and Technology [2011225]
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A DNAzyme based method for the sensitive and selective quantification of lead(II) ions has been developed. A DNAzyme that requires Pb2+ for activation was selected. An RNA containing DNA substrate was cleaved by the DNAzyme in the presence of Pb2+. The 2',3'-cyclic phosphate of the cleaved 5'-part of the substrate was efficiently removed by Exonuclease III. The remaining part of the single stranded DNA (9 or 13 base long) was subsequently used as the primer for the strand displacement amplification reaction (SDAR). The method is highly sensitive, 200 pM lead(II) could be easily detected. A number of interference ions were tested, and the sensor showed good selectivity. Underground water samples were also tested, which demonstrated the feasibility of the current approach for real sample applications. It is feasible that our method could be used for DNAzyme or aptazyme based new sensing method developments for the quantification of other target analytes with high sensitivity and selectivity. (C) 2013 Elsevier B.V. All rights reserved.
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