Journal
BIOSENSORS & BIOELECTRONICS
Volume 54, Issue -, Pages 222-228Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.11.017
Keywords
Hydrogel; DNA biosensor; Electrochemical quartz crystal microbalance; Electrochemical impedance spectroscopy; Scanning electron microscopy
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Funding
- Polish Ministry of Science and Higher Education [IP2011 025971]
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Application of hydrogel based on N-isopropylacrylamide with carboxyl groups grafted to the chains enabled the immobilization of DNA at an extent exceeding that for flat surfaces by at least one order of magnitude. The probe DNA strands in the 3D platform were fully available for the hybridization process. The examination of the gels containing different amounts of grafted carboxyl groups (1-10%) was done using quartz crystal microbalance, electrochemical impedance spectroscopy, chronoamperometry and ionic coupled plasma with laser ablation. The optimal carboxyl group content was determined to be 5%. A very good agreement of the data obtained with independent techniques on content of DNA in the gel was obtained. In comparison to the other methods of immobilization of DNA the new platform enabled complete removal of DNA after the measurements and analysis and, therefore, could be used many times. After a 10-fold exchange of the DNA-sensing layer the efficiency of hybridization and analytical signal did not change by more than 5%. The sensor response increased linearly with logarithm of concentration of target DNA in the range 1 x 10(-13)-1 x 10(-6) M. The obtained detection limit was circa 8 x 10-13 M of target DNA in the sample which is a substantial improvement over the planar sensing layers. (C) 2013 Elsevier B.V. All rights reserved.
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