Journal
BIOSENSORS & BIOELECTRONICS
Volume 52, Issue -, Pages 298-303Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.09.010
Keywords
Visual probe; Red-emitting fluorescence; Fluoride; Ratiometric detection; Bioimaging
Categories
Funding
- National Natural Science Foundation of China [21107029, 21175057, 21075052]
- National Major Projects on Water Pollution Control and Management Technology [2008ZX07422]
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Recently, growing attention has been paid to the accurate determination of fluoride anion (F-) in the environment and living systems for its toxicity and biological function investigation. In this paper, we developed a ratiometric visual and red-emitting fluorescent dual-channel probe (1) employed Si-O bond as a highly selective recognition receptor for imaging F- in living cells. Probe 1 possesses a potential internal charge transfer (ICT) structure, and displays a large (158 nm) red-shifted absorption spectrum and the color changes from yellow to blue upon addition of F- in the aqueous solution. In addition, probe 1 can be used to detect F- quantitatively by the ratiometric absorption and turn-on fluorescence spectroscopy methods with excellent sensitivity. Finally, the results of its application to bioimaging of F- in living cells show that probe 1 would be of great benefit to biomedical researchers for investigating the effects of fluoride in biological systems. (C) 2013 Elsevier B.V. All rights reserved.
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