Journal
BIOSENSORS & BIOELECTRONICS
Volume 40, Issue 1, Pages 417-421Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.07.020
Keywords
NRK cells; Volume change; Osmotic stress; Surface plasmon resonance; Long-range surface plasmon
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Cell volume and its regulation are key factors for cellular integrity and also serve as indicators of various cell pathologies. SPR sensors represent an efficient tool for real-time and label-free observations of changes in cell volume and shape. Here, we extend this concept by employing the use of long-range surface plasmons (LRSP). Due to the enhanced penetration depth of LRSP (similar to 1 mu m, compared to similar to 0.4 mu m of a conventional surface plasmon), the observation of refractive index changes occurring deeper inside the cells is possible. In this work, the responses of a confluent normal rat kidney (NRK) epithelial cell layer to osmotic stress are studied by both conventional and long-range surface plasmons. Experiments are conducted in parallel using cell layers grown and stimulated under the same conditions to enable direct comparison of the results and discrimination of the osmotic stress-induced effects in different parts of the cell. (C) 2012 Elsevier B.V. All rights reserved.
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