4.8 Article

Quartz crystal microbalance with dissipation (QCM-D) as tool to exploit antigen-antibody interactions in pancreatic ductal adenocarcinoma detection

Journal

BIOSENSORS & BIOELECTRONICS
Volume 42, Issue -, Pages 646-652

Publisher

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.10.012

Keywords

Quartz crystal microbalance; Self-assembled monolayer; alpha-enolase; Pancreatic ductal adenocarcinoma

Funding

  1. EU [213803]
  2. regione Puglia: Progetto Strategico [PS105]
  3. Progetto Reti di Laboratori (NaBiDit)
  4. European Community [256974]
  5. Associazione Italiana Ricerca sul Cancro (AIRC) [12182]
  6. IG [5548, 11643]
  7. Ministero della Salute: Progetto Integrato Oncologia
  8. Regione Piemonte: Ricerca Industriale e Sviluppo Precompetitivo (BIOPRO and ONCOPROT)
  9. Ricerca Industriale Converging Technologies (BIOTHER)
  10. Progetti strategici su tematiche di interesse regionale o sovra regionale (IMMONC)
  11. Ricerca Sanitaria Finalizzata, Ricerca Sanitaria Applicata
  12. Ministero dell'Istruzione e della Ricerca (MIUR)
  13. Progetti di Rilevante Interesse Nazionale (PRIN)
  14. University of Turin-Progetti di Ateneo
  15. Mechanisms of REsistance to anti-angiogenesis regimens THErapy [Rethe-ORTO11RKTW]
  16. Fondazione Italiana Ricerca sul Cancro (FIRC)

Ask authors/readers for more resources

Novel synthetic peptides represent smart molecules for antigen antibody interactions in several bioanalytics applications, from purification to serum screening. Their immobilization onto a solid phase is considered a key point for sensitivity increasing. In this view, we exploited Quartz Crystal Microbalance with simultaneous frequency and dissipation monitoring (QCM-D) with a double aim, specifically, as investigative tool for spacers monolayer assembling and its functional evaluation, as well as high sensitive method for specific immunosorbent assays. The method was applied to pancreatic ductal adenocarcinoma (PDAC) detection by studying the interactions between synthetic phosphorylated and un-phosphorylated alpha-enolase peptides with sera of healthy and PDAC patients. The synthetic peptides were immobilized on the gold surface of the QCM-D sensor via a self-assembled alkanethiol monolayer. The presented experimental results can be applied to the development of surfaces less sensitive to non-specific interactions with the final target to suggest specific protocols for detecting PDAC markers with un-labeled biosensors. (C) 2012 Elsevier B.V. All rights reserved.

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