Journal
BIOSENSORS & BIOELECTRONICS
Volume 41, Issue -, Pages 621-626Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.09.040
Keywords
Reduced graphene oxide; Field-effect transistor; Biosensor; Immunosensing; Label-free detection
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) [2010-0015035]
- WCU Program through the National Research Foundation of Korea (NRF) [R32-2008-000-10124-0]
- Ministry of Education, Science and Technology (MEST)
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We report reduced graphene oxide field effect transistor (R-GO FET) biosensor for label-free ultrasensitive detection of a prostate cancer biomarker, prostate specific antigen/alpha 1-antichymotrypsin (PSA-ACT) complex. The R-GO channel in the device was formed by reduction of graphene oxide nanosheets networked by a self-assembly process. Immunoreaction of PSA-ACT complexes with PSA monoclonal antibodies on the R-GO channel surface caused a linear response in the shift of the gate voltage, V-g,V-min, where the minimum conductivity occurs. The R-GO FET can detect protein-protein interactions down to femtomolar level with a dynamic range over 6-orders of magnitude in the V-g,V-min shift as a sensitivity parameter. High association constants of 3.2 nM(-1) and 4.2 nM(-1) were obtained for the pH 6.2 and pH 7.4 analyte solutions, respectively. The R-GO FET biosensor showed a high specificity to other cancer biomarker in the phosphate buffered saline solutions as well as in the human serum. (C) 2012 Elsevier B.V. All rights reserved.
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