Journal
BIOSENSORS & BIOELECTRONICS
Volume 44, Issue -, Pages 115-121Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.12.046
Keywords
Exosome; Electrochemical sensors; Tumor biomarkers; Lung cancer; Salivary diagnostics
Categories
Funding
- National Center for Research Resources
- National Center for Advancing Translational Sciences, National Institutes of Health [UL1TR000124]
- Felix & Mildred Yip Endowed Professorship
- Barnes Family Fund
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Exosomes biomarkers mediating important biological process, especially in the systemic disease diagnostics and therapeutics, yet the protective exosomal vesicle structure hinders rapid, simple detection of the harbored molecules. We have established a new method, the electric field-induced release and measurement (EFIRM), which can simultaneously disrupt exosomes to release the contents and on-site monitoring the harbored exosomal RNA/proteins biomarkers. When exposed to a non-uniform electrical field, exosomal RNA and proteins are rapidly released. Bio-recognition of these biomolecules is carried out concurrently. We tested the hypothesis that the lung cancer cell line, H460 stably transfected with hCD63-GFP, would shed hCD63-GFP expressing exosomes that could be detected in serum and saliva. We confirmed in vivo that H460-CD63-GFP shed exosomes were transported to blood and saliva. This result demonstrates for the first time tumor-shed exosomes were detected in saliva, in addition to blood, presenting a new translational utility of exosome-based biomarker detection in saliva. (C) 2013 Elsevier B.V. All rights reserved.
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