Journal
BIOSENSORS & BIOELECTRONICS
Volume 47, Issue -, Pages 278-284Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.03.031
Keywords
Bacterial chemotaxis; Pump-less microfluidic device; Diffusion; Switching motility; Pseudomonas aeruginosa
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Funding
- Converging Research Center Program through the Ministry of Education, Science and Technology [2012K001490]
- National Research Foundation of Korea (NRF)
- Korean government (MEST) [2012-0005678]
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Bacterial chemotaxis is a complex response to temporal changes of concentrations of chemoeffectors. Conventional methods, including microfluidic approaches, for the precise analysis of bacterial chemotaxis are limited in the accurate control of chemical gradients, low sensitivity, and longer analytical times although the advances in microfluidic technology provide a novel platform. Here, we present a diffusion-based microfluidic device to provide rapid diffusion of chemoeffectors using a liquid-liquid interface, which is a critical advance in the analysis of bacterial chemotaxis. The microfluidic method can rapidly analyze attractants and repellents, achieving a chemotaxis index corresponding to the concentrations and types of chemoeffectors. Moreover, we find that the dynamic switch in bacterial chemotaxis from attraction to repellence in response to specific chemoeffectors occurs in a concentration-dependent manner. Thus, our microfluidic method is promising and reliable for the analysis of bacterial chemotaxis. (C) 2013 Elsevier B.V. All rights reserved.
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