Journal
BIOSENSORS & BIOELECTRONICS
Volume 45, Issue -, Pages 230-236Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.02.008
Keywords
Graphene oxide; Gold nanorod; Surface plasmon resonance; Transferrin
Categories
Funding
- National Natural Science Foundation of China [20727003, 21075049, 21105037]
- Program for New Century Excellent Talents in University [NECT-10-0443]
- Special-funded Programme on National Key Scientific Instruments and Equipment Development [2012YQ090194]
- Science and Technology Developing Foundation of Jilin Province [20100356, 20110162]
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A surface plasmon resonance (SPR) biosensor based on graphene oxide (GO) decorated with gold nanorod (AuNR)-antibody conjugates was developed. Compared with traditional SPR biosensor, GO sheets were assembled on the amino-modified Au film via electrostatic interaction, and then AuNR-antibody conjugates were immobilized via carbodiimide-assisted amidation reaction. The abundant oxygen-containing functional groups, large specific surface area and friendly biocompatibility of GO sheets are beneficial to the immobilization of AuNR-antibody conjugates. Meanwhile, AuNRs are anchored to the GO surface through antibody and function as enhancers for the transferrin determination, which greatly enhance the sensitivity of the detection. As a result, the present biosensor shows a satisfactory response to transferrin in the concentration range of 0.0375-40.00 mu g mL(-1). The lowest concentration of transferrin that can be determined by this method is about 32-fold lower than that obtained with the sensor based on Au film. This design affords a facile method of controlling the assembly of AuNRs on the GO sheets and can be easily extended to other protein detection by preparing corresponding AuNR-antibody conjugates. (C) 2013 Elsevier B.V. All rights reserved.
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