Journal
BIOSENSORS & BIOELECTRONICS
Volume 39, Issue 1, Pages 244-249Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.07.054
Keywords
Trimethylamine; Carbon nanotube; Field-effect transistor; Seafood quality; Olfactory receptor; Bioelectronic nose
Categories
Funding
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology (MEST)
- NRF [2011-0020984, 2012-0000144, 2012K001365, 2012-0000117, 2012K001366]
- National Research Foundation of Korea [2009-0079103, 2010-50233, 2010-0020821, 2009-0080242, 2010-50232] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We herein report a peptide receptor-based bioelectronic nose (PRBN) that can determine the quality of seafood in real-time through measuring the amount of trimethylamine (TMA) generated from spoiled seafood. The PRBN was developed using single walled-carbon nanotube field-effect transistors (SWNT-FETs) functionalized with olfactory receptor-derived peptides (ORPs) which can recognize TMA and it allowed us to sensitively and selectively detect TMA in real-time at concentrations as low as 10 fM. Utilizing these properties, we were able to not only determine the quality of three kinds of seafood (oyster, shrimp, and lobster), but were also able to distinguish spoiled seafood from other types of spoiled foods without any pretreatment processes. Especially, the use of small synthetic peptide rather than the whole protein allowed PRBNs to be simply manufactured through a single-step process and to be reused with high reproducibility due to no requirement of lipid bilayers. Furthermore, the PRBN was produced on a portable scale making it effectively useful for the food industry where the on-site measurement of seafood quality is required. (C) 2012 Elsevier B.V. All rights reserved.
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