Journal
BIOSENSORS & BIOELECTRONICS
Volume 41, Issue -, Pages 704-709Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.09.053
Keywords
Fluorescent aptasensor; Label-free; OTA; Tb3+; Structure-switching; MBs
Categories
Funding
- National Natural Science Foundation of China [21105012, 21205015]
- Program for New Century Excellent Talents in University of China [NCET-10-0010]
- Agricultural Five New Projects of Fujian Development and Reform Commission
- National Science Foundation of Fujian Province [2011J01028]
- Program for Fujian University Outstanding Youth Scientific Research [JA11105, JA10295]
- Youth Foundation of Fujian Health Department [2010126]
- Foundation of Medical Innovation of Fujian Health Department [2011-CX-22]
- Foundation of Fuzhou Science and Technology Bureau [2012-S-148]
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On the basis of Tb3+, structure-switching aptamer and magnetic beads (MBs), a signal-on fluorescent aptasensor was developed for the label-free determination of OTA in wheat. Initially, the specific sequence of the anti-OTA aptamer labeled with a biotin group, was attached to streptavidin-modified MBs. Two single-stranded signal probes were added and naturally hybridized with anti-OTA aptamer to form the duplex structure in the solution. Due to the fact that single-stranded oligonucleotides can greatly enhance the emission of Tb3+ in solution but duplexes do not, through magnetic separation, the supernatant liquid of the above solution contained no single-stranded DNA and cannot increase the emission of Tb3+. While upon OTA addition, it will bind with aptamer to form OTA-aptamer G-quadruplex while releasing two single-stranded signal probes. Through magnetic separation, the released single-stranded signal probes left in the supernatant liquid can dramatically increase the fluorescent intensity of Tb3+. By employing the above strategy, this aptasensor can detect as low as 20 pg/mL OTA with high specificity. To the best of our knowledge, the proposed aptasensor is the first attempt to use the fluorescent characteristics of Tb3+ for OTA detection, which may represent a promising path toward routine quality control of food safety. (C) 2012 Elsevier B.V. All rights reserved.
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