An aptamer based surface plasmon resonance biosensor for the detection of bovine catalase in milk

In this research, we report the development of an aptamer based SPR biosensor for the detection of catalase in milk samples with minimal sample preparation. A biotin tagged aptamer was immobilized onto a gold surface by affinity capture. A limit of detection (LOD) in the nanomolar range (20.5 nM, RSD: 15.2%) was found and a dynamic range of 15-1000 nM was established for catalase in buffer and the aptamer showed good specificity toward catalase. This biosensor has the potential to be used in the detection of catalase in milk samples, a key indicator of mastitis disease in milk. (C) 2013 Elsevier B.V. All rights reserved.