Journal
BIOSENSORS & BIOELECTRONICS
Volume 39, Issue 1, Pages 320-323Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.07.026
Keywords
Layered double hydroxides; Acetylcholinesterase; Flow injection/amperometric analysis; Methyl parathion; Biosensor
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Funding
- National Science Foundation of China [20803026, 21175053]
- Program for Chenguang Young Scientist for Wuhan [201271031412]
- CCNU from the Colleges' Basic Research and Operation of MOE [CCNU10A01006]
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We developed a highly sensitive flow injection/amperometric biosensor for the detection of organophosphate pesticides (OPs) using layered double hydroxides (LDHs) as the immobilization matrix of acetylcholinesterase (AChE). LDHs provided a biocompatible microenvironment to keep the bioactivity of AChE, due to the intrinsic properties of LDHs (such as a regular structure, good mechanical, chemical and thermal stabilities, and swelling properties). By integrating the flow injection analysis (FIA) with amperometric detection, the resulting AChE-LDHs modified electrode greatly catalyzed the oxidation of the enzymatically generated thiocholine product, and facilitated the detection automation, thus increasing the detection sensitivity. The analytical conditions for the FIA/amperometric detection of OPs were optimized by using methyl parathion (MP) as a model. The inhibition of MP was proportional to its concentration ranging from 0.005 to 0.3 mu g mL(-1) and 0.3 to 4.0 mu g mL(-1) with a detection limit 0.6 ng mL(-1) (S/N=3). The developed biosensor exhibited good reproducibility and acceptable stability. (C) 2012 Elsevier B.V. All rights reserved.
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