Journal
BIOSENSORS & BIOELECTRONICS
Volume 50, Issue -, Pages 387-392Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.06.055
Keywords
Graphene; Micropilar; Electrochemical sensor; Phenol detection; Enzyme
Categories
Funding
- Converging Technology Project
- Korean Ministry of Environment [M112-00061-0002-0]
- Basic Science Research Program
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2012-0004343, 2011K000837]
- Converging Research Center Program
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A three-dimensional (3D) graphene incorporated electrochemical sensor was constructed for sensitive enzyme based phenol detection. To form the 3D graphene structure, polydimethylsiloxane (PDMS) micropillars were fabricated in the microchannel by using a conventional photolithography and the surface was modified with 3-aminopropyltriethoxysilane. Then, the negatively charged graphene oxide sheets were electrostatically adsorbed on the PDMS micropillar surface, and reduced in the hydrazine vapor. The resultant 3D graphene film provides a conductive working electrode as well as an enzyme-mediated sensor with a large surface area. After bonded with an electrode patterned glass wafer, the 3D graphene based electrochemical sensor was produced. Using the 3D graphene as a working electrode, an excellent electron transfer property was demonstrated by cyclic voltammetry measurement in an electrolyte solution containing 1 mM K3Fe(CN)(6) and 0.1 M KCI. To utilize the 3D graphene as an enzyme sensor, tyrosinase enzymes were immobilized on the surface of the graphene micropillar, and the target phenol was injected in the microchannel. The enzyme catalytic reaction process was monitored by amperometric responses and the limit of detection for phenol was obtained as 50 nM, thereby suggesting that the 3D graphene micropillar structure enhances the enzyme biosensing capability not only by increasing the surface area for enzyme immobilization, but also by the superlative graphene conductivity property. (C) 2013 Published by Elsevier B.V.
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