Journal
BIOSENSORS & BIOELECTRONICS
Volume 41, Issue -, Pages 532-537Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.09.016
Keywords
Magnetic bead concentration; Bacteria detection; Nanoporous membrane; Impedance sensor
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Funding
- Hong Kong Research Council General Research Grant [PolyU 5358/09E]
- Hong Kong Ph.D. Fellowship Scheme Fund [HKPF10-13386/ PolyU RUY5]
- Research Grant Council Direct Allocation Fund [A-PK51]
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In this paper, biofunctional magnetic beads were investigated for bacterial cells concentration in a nanoporous alumina membrane based immunosensor for ultra-sensitive detection of E. coli O157:H7. The specific antibody modified magnetic beads were used for concentration of E coli O157:H7 from samples in a small region to enhance sensitivity. The magnetic bead conjugated E. coli O157:H7 cells were then captured on the nanoporous alumina membrane with immobilized antibody via assembled PEG-silane linker. Scanning electron microscopy and fluorescent microscopy were used to demonstrate the magnetic bead-bacteria cell conjugation and bacteria cells magnetic concentration, respectively. Impedance spectroscopy was used to monitor the pure E con O157:H7 cells and magnetic bead conjugated E coli O157:H7 cells binding on antibody immobilized nanoporous membrane with or without magnetic field. Compared with direct detection of pure bacteria cells, this method via magnetic bead conjugation and concentration demonstrated the ultrasensitivity of 10 CFU/mL for E coli O157:H7 detection. (C) 2012 Elsevier B.V. All rights reserved.
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