Journal
BIOSENSORS & BIOELECTRONICS
Volume 47, Issue -, Pages 502-507Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.03.057
Keywords
C-60-carboxyfullerenes; Peroxidase-like activity; Colorimetric sensor; Glucose
Categories
Funding
- 973 Program [2011CB302100]
- National Natural Science Foundation of China [21121063, 91027018, 51172244]
- NSAF [11076027]
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C-60-carboxyfullerene, C-60[C(COOH)(2)](2), has been proven to function as peroxidase mimetics that can catalyze the reaction of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a blue color reaction. Kinetic studies further indicate that C-60[C(COOH)(2)](2) has an even higher affinity to TMB than that of the natural enzyme, horseradish peroxidase (HRP). C-60[C(COOH)(2)](2)/glucose oxidase (GO(x))/TMB system provides a novel colorimetric sensor for glucose and shows good response toward glucose detection over a range of 1.0-40 mu M with a limit of detection 0.5 mu M (3 sigma/slope). Moreover, this sensitive and selective sensor can be successfully applied for the quantitative determination of glucose in human serum. The results indicate that it is a simple, cheap, convenient, highly selective, sensitive, and easy handling colorimetric assay. (C) 2013 Elsevier B.V. All rights reserved.
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