Journal
BIOSENSORS & BIOELECTRONICS
Volume 50, Issue -, Pages 338-344Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.06.037
Keywords
Antigen microarray; Polymer brush; Competitive immunoassay; Mycotoxins
Categories
Funding
- National Program on Key Basic Research Project of China (973 Program) [2013CB127804]
- National Natural Science Foundation of China [21205098, 21273173, 31171702]
- Special Fund for Agro-scientific Research in the Public Interest [201203094]
- Institute for Clean Energy & Advanced Materials (Southwest University, Chongqing, China)
- Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies
- Southwest University [SWU111071]
- Chongqing Engineering Research Center for Rapid diagnosis of Dread Disease and Chongqing Development and Reform Commission, China
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Various mycotoxins with strong carcinogenesis and toxicity are fatal threats in food safety, and require highly sensitive and high-throughput detections greatly. Herein a fluorescent competitive immunoassay microarray based on a non-fouling polymer brush, poly[(ethylene glycol) methacylate-co-glycidyl methacrylatej (POEGMA-co-GMA) is explored to sensitively detect multiple mycotoxins with aflatoxin B1 (AFB1), ochratoxin A (OTA) and zearalenone (ZEN) as template targets. Due to uniformly large protein loading and high resistance to nonspecific protein absorption of the POEGMA-co-GMA brush, the optimal microarray exhibits wide dynamic ranges of three orders of magnitudes and low detection limits of 4, 4 and 3 pg mL(-1) respectively, which is much better than that obtained with an epoxy-functionalized antigen microarray, and is comparable or even better than the conventional ELISA method. This work offers a powerful high-throughput tool to fast screening of toxins in food quality and environmental monitoring. (C) 2013 Elsevier B.V. All rights reserved.
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