Journal
BIOSENSORS & BIOELECTRONICS
Volume 45, Issue -, Pages 102-107Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.01.065
Keywords
Graphene; Electrocatalysis; Electrochemistry; Supramolecular assembly; Cyclodextrin
Categories
Funding
- National Key Scientific Program of China [2011CB911000]
- NSFC [20975034, 21177036]
- Foundation for Innovative Research Groups of NSFC [21221003]
- Natural Science Foundation of Jiangxi Province [20122BAB213007]
- National Instrumentation Program [2011YQ030124]
- Ministry of Education of China [20100161110011]
- Hunan Provincial Natural Science Foundation [11JJ1002]
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The self-assembly of cyclodextrin (CD) functionalized graphene (GR) and adamantane-modified horseradish peroxidase (HRP-ADA) by host-guest supramolecular interaction into novel nanostructures in aqueous solution is reported in the present study. Electrochemical impedance spectroscopy and cyclic voltammetry were applied to characterize the self-assembly process and study the electrochemical behaviors of the immobilized proteins. UV-vis spectra indicated that the native structure of HRP was maintained after the assembly, implying good biocompatibility of CD-functionalized GR (CD-GR). Furthermore, the HRP-ADA/CD-GR composites were utilized for the fabrication of enzyme electrodes (HRP-ADA/CD-GR electrodes). The proposed biosensor showed good reproducibility and high sensitivity to H2O2 with the detection limit of 0.1 mu M. In the range of 0.7-35 mu M, the catalytic reduction current of H2O2 was proportional to H2O2 concentration. (C) 2013 Elsevier B.V. All rights reserved.
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