Journal
BIOSENSORS & BIOELECTRONICS
Volume 50, Issue -, Pages 453-459Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.07.019
Keywords
Electrical measurement; ECIS; Cellular cytotoxicity; Hydrogel; Diffusion; Micro-electrode
Categories
Funding
- National Research Foundation of Korea (NRF)
- Korean Government (MEST) [2013R1A2A2A03015620]
- Basic Science Research Program
- Ministry of Education, Science and Technology [2011-0022060]
- National Research Foundation of Korea [2013R1A2A2A03015620, 2011-0022060] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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In this study, we have provided a novel analytical integration between hydrogel-based cell chip and Electric Cell-substrate Impedance Sensing (ECIS) technique to apply to a high-throughput, real-time cell viability assay and drug screening. For simulating the drug diffusion model, we have developed a hydrogel-based tissue-mimicking structure with microfluidic channel, without unwanted flow, to generate a gradient concentration with long-term stability. Along the gradient line, four individual micro-electrodes were installed to record the impedance signal changes, which result from the cell viability under drug effects. By watching for cellular impedance changes, we successfully estimated the cytotoxicity of the treatment corresponding to the various concentration values of stimuli, generated by the diffusion process along the channel. Reliable IC50 values and time-dose relationships were also achieved. With the feature of real-time monitoring capability, the advantages of non-invasion, label-free detection, time saving and simple manipulation, our integrative device has become a promising high throughput cell-based on-chip platform for cell viability assay and drug screening. (C) 2013 Elsevier B.V. All rights reserved.
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