Journal
EXPERIMENTAL AND MOLECULAR MEDICINE
Volume 50, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s12276-018-0060-y
Keywords
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Funding
- NSF [MCB-1616332]
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Cancer metabolism is significantly altered from normal cellular metabolism allowing cancer cells to adapt to changing microenvironments and maintain high rates of proliferation. In the past decade, stable-isotope tracing and network analysis have become powerful tools for uncovering metabolic pathways that are differentially activated in cancer cells. In particular, C-13 metabolic flux analysis (C-13-MFA) has emerged as the primary technique for quantifying intracellular fluxes in cancer cells. In this review, we provide a practical guide for investigators interested in getting started with C-13-MFA. We describe best practices in C-13-MFA, highlight potential pitfalls and alternative approaches, and conclude with new developments that can further enhance our understanding of cancer metabolism.
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