Journal
BIOSENSORS & BIOELECTRONICS
Volume 44, Issue -, Pages 85-88Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.01.011
Keywords
Graphene; Silk peptide; Tyrosinase; Biosensor; Phenolic compounds
Categories
Funding
- Natural Science Foundation of China [21275166, 21275165, 21275167]
- Wuhan Science and Technology Bureau [201160723224]
- Research foundation of State General Administration of The People's Republic of China for Quality Supervision and Inspection and Quarantine [2011IK217, 2012IK208]
- Natural Science Foundation of Guangxi province [2010GXNSFA013061]
Ask authors/readers for more resources
New graphene-silk peptide (Gr-SP) nanosheets were prepared and successfully fabricated with tyrosinase (Tyr) as a novel biosensor for the determination of phenolic compounds. The Gr-SP nanosheets were fully characterized with transmission electron microscopy, x-ray diffraction, x-ray photoelectron spectroscopy, UV/Vis and FTIR spectra. The developed biosensors were also characterized with scanning electronic microscopy and electrochemical impedance spectroscopy. Using bisphenol A (BPA) as a model substrate in the sensing system, a number of key factors including the volume of Gr-SP-Tyr solution, the applied potential, pH values, temperature, and the Tyr/Gr-SP ratio that influence the analytical performance of the biosensor were investigated. The biosensor gave a linear response on the concentration ranges of 0.001-16.91 mu M for catechol with the sensitivity of 7634 mA M-1 cm(-2), 0.0015-21.12 mu M for phenol with the sensitivity of 4082 mA M-1 cm(-2), and 0.002-5.48 mu M for BPA with the sensitivity of 2511 mA M-1 cm(-2). The low detection limits were estimated to be 0.23, 035 and 0.72 nM (S/N=3) for catechol, phenol and BPA, respectively. The biosensors also exhibit good repeatability and long-term stability. The practical application of the biosensor was also demonstrated by the determination of BPA leaching from commercial plastic drinking bottles. (C) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available