Journal
JOURNAL OF CHROMATOGRAPHIC SCIENCE
Volume 53, Issue 3, Pages 436-442Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/chromsci/bmu225
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Funding
- Hungarian National Granting Office, Budapest, Hungary [OTKA 100155]
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Mobile phase progress in planar stationary phase can be evoked by either external or internal forces. An internal force is capillarity, while gravity, electric field, a pump and centrifugal forces belong to external forces. Overpressured layer chromatography gives a widely used special chapter of forced-flow planar chromatography, a special bridge between high-performance liquid column chromatography and thin-layer chromatography (TLC). A simple and special rule characterizes the progress of mobile phase. Optimal efficiency is composed by the doubled effect of flow resulting from the pump-forced mobile phase (convex profile of laminar flow) and capillary forces on the dry stationary phase (concave laminar flow). This review describes the most important aspects of forced-flow TLC, including how the setups are developed and also the progress of detection methods used.
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