Journal
BIOSENSORS & BIOELECTRONICS
Volume 41, Issue -, Pages 880-883Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.09.015
Keywords
Dynamic light scattering (DLS); DNA; Gold nanoparticles (AuNPs); Glucose; Glucose oxidase (GOD); Fenton reaction
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Funding
- National Natural Science Foundation of China (NSFC) [20975116, 50830107]
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Dynamic light scattering based sensor for glucose was developed with oligonucleotide functionalized gold nanoparticles (Oligo-AuNPs). Oligonucleotide 5'-SH-(A)(12)-AGACAAGAGAGG-3' (Oligo 1) modified AuNPs and oligonucleotide 5'-CAACAGAGAACG-(A)(12)-HS-3' (Oligo 2) modified AuNPs could hybridize with oligonulceotide 5'-CGTTCTCTGTTGCCTCTCTTGTCT-3' (Oligo 3), which resulted in the aggregation of Oligo-AuNPs probes, and triggered the increase of their average diameter. However, Oligo 3 could be cleaved into DNA fragments by the mixture of glucose, glucose oxidase (GOD) and Fe2+, and the DNA fragments could not hybridize with Oligo-AuNPs probes. Under the conditions of 3.7 nM Oligo 1-AuNPs, 3.7 nM Oligo 2-AuNPs, 8.0 mu g/mL GOD, 100 nM Oligo 3 and 900 nM Fe2+, the average diameter of Oligo-AuNPs probes decreased linearly with the increasing concentration of glucose over the range from 50 pmol/L to 5.0 nmol/L, with a detection limit of 38 pmol/L (3 sigma/slope). Moreover, five sugars had no effect on the average diameter of mixture of Oligo-AuNPs probes, GOD and Fe2+, which demonstrated the good selectivity of the assay. (C) 2012 Elsevier B.V. All rights reserved.
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