Journal
BIOSENSORS & BIOELECTRONICS
Volume 48, Issue -, Pages 19-25Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.03.083
Keywords
Upconversion nanoparticles; Au nanorods; FRET; Thrombin; Aptamer sensor
Categories
Funding
- Natural Science Foundation of China [21075002, 21245008, 20905003]
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In this work, we design a FRET system for sensitive and selective determination of thrombin in red region, in which NaYF4:Yb,Er upconversion nanoparticles (UCNPs) act as donor and gold nanorods (Au NRs) act as acceptor. NaYF4:Yb,Er UCNPs with a strong emission at 661 nm were successfully synthesized by tuning the doped ions ratio. Carboxyl-functionalized NaYF4:Yb,Er UCNPs and Au NRs were then prepared and conjugated with the thrombin aptamers, respectively. The fluorescence emission band of NaYF4:Yb,Er UCNPs (lambda(max) = 661 nm) highly overlaps with the absorption band of Au NRs(lambda(max) = 666 nm), which benefits from the large tunability of the spectrum band of Au NRs. A FRET system was then formed when thrombin was added to the mixture of NaYF4:Yb,Er UCNPs and Au NRs, which were both modified thrombin aptamers. The fluorescence quenching efficiency of NaYF4:Yb,Er UCNPs was increased in a thrombin concentration-dependent manner, which built the principle of thrombin quantification. The linear range was 2.5-90 nM in an aqueous buffer, and 3.75-112.5 nM in spiked human serum samples for thrombin. It also demonstrates a high selectivity to other biological species due to the specific binding. The measurement of thrombin in human plasma is satisfying, suggesting that the FRET system is of practical value in a complex biological sample matrix in red region. (C) 2013 Elsevier B.V. All rights reserved.
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