Journal
BIOSENSORS & BIOELECTRONICS
Volume 47, Issue -, Pages 113-119Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.03.017
Keywords
Microfluidics; Cancer cells; Deterministic lateral displacement; Antibody
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Funding
- National Natural Science Foundation of China [61106128]
- Knowledge Innovation Project of the Chinese Academy of Science [KGCX2-YW-904]
- Guangdong Innovation Research Team Fund for Low-cost Healthcare Technologies (GIRTF-LCHT)
- ShenZhen Development and Reform Commission Project [2012-385]
- ShenZhen Science and Technology Plan Project [201101004]
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In this work, we introduced an integrated microfluidic system for fast and efficient circulating tumor cell (CTC) isolation and capture. In this microfluidic platform, a combination of microfluidic deterministic lateral displacement array and affinity-based cell capture architecture, allows for the high efficiency cancer cell enrichment and continuous high throughput and purity cancer cell capture. Using this device to isolate breast cancer cells from spiked blood samples, we achieved an enrichment factor of 1500 x, and a high processing throughput of 9.6 mL/min with 90% capture yield and more than 50% capture purity at cell concentration 10(2) cells/mL. This integrated platform offers a promising approach for CTC capture with high recovery rates, purity and stability, and exhibits potential capability in cancer cell culture and drug screening. (C) 2013 Elsevier B.V. All rights reserved.
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