Journal
BIOSENSORS & BIOELECTRONICS
Volume 50, Issue -, Pages 42-49Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.05.060
Keywords
Fluorescent pH probe; Ratiometric; Two-photon; Bioimaging; Acidic compartments
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Funding
- National Natural Science Foundation of China [51273107]
- Natural Science Foundation of Shandong Province, China [ZR2012EM001]
- Shandong University [2011JC006]
- Open Project of State Key Laboratory for Supramolecular Structure and Materials [SKLSSM201207]
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Fluorescent imaging of acidic compartments in living cells was carried out successfully by a novel molecule (CAE) that contained a pyridine unit and a carbazole core. As the protonation of the pyridine N atom of CAE, pH-dependent absorption and fluorescence properties were shown with a pK(a) of 5.47 which matches the pH range of intracellular acidic compartments. Moreover, a large Stokes shift, a significant enhancement in ratios of I-544 nm/I-460 (nm) and a distinct two-photon turn-on character were exhibited in spectral analysis. Meanwhile, direct intracellular imaging and standard double-staining experiments of CAE and LTR (co-localization coefficient: 0.83) revealed that CAE is an effective one-photon ratiometric and two-photon acidic pH probe for imaging intracellular acidic compartments. The pH distribution pattern of intracellular acidic compartments can be obtained facilely by CAE. In especial, CAE possessed well membrane-permeability, brilliant selectivity among various bioanalyte and excellent counterstain compatibility with Hoechst 33342, MTR and LTR. (C) 2013 Elsevier B.V. All rights reserved.
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