Journal
BIOSENSORS & BIOELECTRONICS
Volume 39, Issue 1, Pages 236-243Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.07.080
Keywords
Molecularly imprinted polymer; Porogen-water; Bovine serum albumin; Multiwalled carbon nanotubes-ceramic electrode; Differential pulse voltammetry
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Funding
- Council of Scientific and Industrial Research-University Grant Commission (CSIR-UGC), New Delhi
- CSIR
- Department of Science and Technology, New Delhi [SR/S1/IC-30/2010]
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This study describes the synthesis of a new class of substrate-selective molecularly imprinted polymer. This involved tetraethylene glycol 3-morpholin propionate acrylate (functional monomer) and bovine serum albumin (template) for polymerization in aqueous condition, using surface grafting-from approach directly on a vinyl exposed multiwalled carbon nanotubes-ceramic electrode. The analyte recapture at pH 6.8 in aqueous environment simultaneously involved hydrophobically driven hydrogen bonds and ionic interactions between negatively charged bovine serum albumin and positively charged imprinted nanofilm. The selectively encapsulated bovine serum albumin first gets reduced at -0.9 V and then oxidized within the cavity, without getting stripped off, to respond a differential pulse voltammetry signal. The limit of detection [0.42 ng mL(-1) (3 sigma, RSD <= 1.02%)] obtained was free from any cross-reactivity and matrix complications in aqueous, pharmaceutical, serum, and liquid milk samples. The proposed sensor can be used as a practical sensor for ultra-trace analysis of bovine serum albumin in clinical settings. (C) 2012 Elsevier B.V. All rights reserved.
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