Journal
BIOSENSORS & BIOELECTRONICS
Volume 35, Issue 1, Pages 308-312Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.03.006
Keywords
Bacteriophages; Long period fiber grating; Spectral interrogation; Pathogen; Covalent binding
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada
- Canada Research Chairs Program
- NanoQuebec
Ask authors/readers for more resources
In this paper we report a stable, label-free, bacteriophage-based detection of Escherichia coli (E. coli) using ultra sensitive long-period fiber gratings (LPFGs). Bacteriophage T4 was covalently immobilized on optical fiber surface and the E. coli binding was investigated using the highly accurate spectral interrogation mechanism. In contrast to the widely used surface plasmon resonance (SPR) based sensors, no moving part or metal deposition is required in our sensor, making the present sensor extremely accurate, very compact and cost effective. We demonstrated that our detection mechanism is capable of reliable detection of E. colt concentrations as low as 10(3) cfu/ml with an experimental accuracy greater than 99%. (C) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available