Enzymatic glucose biosensor based on CeO2 nanorods synthesized by non-isothermal precipitation

Cerium oxide nanorods (CeO2 NRs) were synthesized without templates through a low cost and simple non-isothermal precipitation method. The structure and morphology of CeO2 NRs were characterized by X-ray diffraction and transmission electron microscopy. The CeO2 NRs films, deposited on indium tin oxide (ITO)-coated glass substrates through electrophoretic deposition, were used for the immobilization of glucose oxidase (GOx). Field emission scanning electron microscopy. Fourier transform infrared spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy were used to characterize the CeO2 NRs/ITO and GOx/CeO2 NRs/ITO electrodes. The GOx/CeO2 NRs/ITO electrode exhibits a linear range for the detection of glucose from 2 to 26 mM (correlation coefficient: 0.99) at 1-2 s response time. Biosensor sensitivity is 0.165 mu A mM(-1) cm(-2) with 100 mu M detection limit. The anti-interference ability of the biosensor was also examined. The mediator-less application of CeO2 NRs for glucose sensing was demonstrated. (C) 2011 Elsevier B.V. All rights reserved.