Journal
BIOSENSORS & BIOELECTRONICS
Volume 33, Issue 1, Pages 44-49Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.12.014
Keywords
Layer-by-layer; Sol-gel; Carbon nanotubes; Gold nanoparticles; Acetylcholine biosensor
Categories
Funding
- National Natural Science Foundation of China [30800261, 81127001]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry [[2009]8]
- Scientific Research Foundation of the State Human Resource Ministry for Returned Chinese Scholars
- Department of Education of Guangdong Province
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A novel acetylcholinesterase (AChE)/choline oxidase (ChOx) bienzyme amperometric acetylcholine biosensor based on gold nanoparticles (AuNPs) and multi-walled carbon nanotubes (MWCNTs) has been successfully developed by self-assembly process in combination of sol-gel technique. A thiolated aqueous silica sol containing MWCNTs and ChOx was first dropped on the surface of a cleaned Pt electrode, and then AuNPs were assembled with the thiolated sol-gel network. Finally, the alternate deposition of poly (diallyldimethylammonium chloride) (PDDA) and AChE was repeated to assemble different layers of PDDA-AChE on the electrode for optimizing AChE loading. Among the resulting biosensors. the biosensor based on two layers of PDDA-AChE multilayer films showed the best performance. It exhibited a wide linear range, high sensitivity and fast amperometric response, which were 0.005-0.4 mM, 3.395 mu A/mM, and within 15s, respectively. The biosensor showed long-term stability and acceptable reproducibility. More importantly, this study could provide a simple and effective multienzyme immobilization platform for meeting the demand of the effective immobilization enzyme on the electrode surface. (C) 2011 Elsevier B.V. All rights reserved.
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